Metabolomic profile of amniotic fluid to evaluate lung maturity: the diaphragmatic hernia lamb model

  • Gloria Pelizzo | g.pelizzo@smatteo.pv.it Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia, Italy.
  • Maurizio Ballico Department of Medical and Biological Sciences, University of Udine, Udine, Italy.
  • Maria Chiara Mimmi Department of Medical and Biological Sciences, University of Udine, Udine, Italy.
  • José Louis Peirò Cincinnati Fetal Center. Pediatric Surgery Division, CCHMC, Cincinnati, OH, United States.
  • Mario Marotta Fetal Surgery Program, Congenital Malformations Research Group, Research Institute of Hospital Universitari Vall d’Hebron, Edifici Infantil, Barcelona, Spain.
  • Costanzo Federico Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia, Italy.
  • Erika Andreatta Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia, Italy.
  • Ghassan Nakib Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia, Italy.
  • Maurilio Sampaolesi Laboratory of Translational Cardiomyology, Stem Cell Interdepartmental Institute, KU Leuven, Belgium; Human Anatomy, University of Pavia, Pavia, Italy.
  • Elisa Zambaiti Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia, Italy.
  • Valeria Calcaterra Department of the Mother and Child Health, Pediatric Surgery Unit, Department of Internal Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia, Italy.

Abstract

Background: Tracheal occlusion (TO) stimulates lung growth in fetuses affected with congenital diaphragmatic hernia (CDH) although the processes involved in lung maturation still remain unknown. The objective of this study was to evaluate the metabolomic profile of amniotic fluid (AF) following TO in fetal lamb model in order to obtain an indirect view of mechanisms involved in pulmonary reversal hypoplasia and biochemical maturity in response to fetal TO.

Methods: Liquid Chromatography Mass Spectrometry was performed on lamb AF samples at: age I (70 days’gestation); age II (102 days’ gestation); age III (136 days’ gestation). CDH was induced at age I and TO at age II.

Results: Betaine, choline, creatinine were found significantly increased during gestation in the control group. The CDH group showed choline (p =0.007) and creatinine (p =0.004) decreases during pregnancy. In the TO group choline and creatinine profiles were restored.

Conclusions: Alveolar tissue and fetal global growth ameliorated after TO. Metabolomics provided useful information on biochemical details during lung maturation. Metabolomic profiling would help to identify the best time to perform TO, in order to increase survival of CDH affected patients.

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Published
2014-11-04
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Original Research Articles
Keywords:
Amniotic fluid, Animal model, Congenital diaphragmatic hernia, Metabolomic, Tracheal occlusion
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How to Cite
Pelizzo, G., Ballico, M., Mimmi, M. C., Peirò, J. L., Marotta, M., Federico, C., Andreatta, E., Nakib, G., Sampaolesi, M., Zambaiti, E., & Calcaterra, V. (2014). Metabolomic profile of amniotic fluid to evaluate lung maturity: the diaphragmatic hernia lamb model. Multidisciplinary Respiratory Medicine, 9(1). https://doi.org/10.4081/mrm.2014.389