DNA damage and repair capacity in lymphocyte of chronic obstructive pulmonary diseases patients during physical exercise with oxygen supplementation
DNA damage and repair capacity in lymphocyte of chronic obstructive pulmonary diseases patients during physical exercise with oxygen supplementation
Authors
Andréa Lúcia Gonçalves da Silva
Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul – UNISC, Bairro Universitário, Santa Cruz do Sul, RS; Hospital Santa Cruz, Santa Cruz do Sul, RS
Thaís Evelyn Karnopp
Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul – UNISC, Santa Cruz do Sul, RS
Augusto Ferreira Weber
Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul – UNISC, Santa Cruz do Sul, RS
Cassia da Luz Goulart
Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul – UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS
Paloma de Borba Scheneiders
Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul – UNISC, Bairro Universitário, Santa Cruz do Sul, RS
Dannuey Machado Cardoso
Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul – UNISC, Bairro Universitário, Santa Cruz do Sul, RS
Lisiane Lisboa Carvalho
Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul – UNISC, Bairro Universitário, Santa Cruz do Sul, RS; Hospital Santa Cruz, Santa Cruz do Sul, RS
Joel Henrique Ellwanger
Programa de Pós-Graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS
Lia Gonçalves Possuelo
Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul – UNISC, Santa Cruz do Sul, RS; Programa de Pós-Graduação em Promoção da Saúde, Universidade de Santa Cruz do Sul – UNISC, Santa Cruz do Sul, RS
Andréia Rosane de Moura Valim
Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul – UNISC, Santa Cruz do Sul, RS; Programa de Pós-Graduação em Promoção da Saúde, Universidade de Santa Cruz do Sul – UNISC, Santa Cruz do Sul, RS
DNA repair, Aerobic exercise, Comet assay, Induced DNA damage, COPD
Abstract
Background: We hypothesized that the use of oxygen supplementation during aerobic exercise induces less DNA damage than exercise alone. The aim of this study is to assess the level of DNA damage induced by physical exercise with and without oxygen supplementation in chronic obstructive pulmonary diseases (COPD) patients. Methods: Peripheral blood was collected before and after aerobic exercise in two conditions: (I) aerobic exercise without oxygen supplementation (AE group) and (II) with oxygen supplementation (AE-O2 group). Lymphocytes were collected to perform the alkaline version of the Comet Assay. To assess the susceptibility to exogenous DNA damage, the lymphocytes were treated with methyl methanesulphonate (MMS) for 1-h or 3-h. After 3-h treatment, the percentage of residual damage was calculated assuming the value of 1-h MMS treatment as 100%. Results: AE group showed lower induced damage (1 h of MMS treatment) and consequently less DNA repair compared to AE-O2 group. AE-O2 group showed an increase in the induced DNA damage (1 h of MMS treatment) and an increased DNA repair capacity. Within the AE-O2 group, in the post-exercise situation the induced DNA damage after 1 h of MMS treatment was higher (p = 0.01) than in the pre-exercise. Conclusion: COPD patients who performed physical exercise associated with oxygen supplementation had a better response to DNA damage induced by MMS and a better DNA repair when compared to the condition of physical exercise without oxygen supplementation. Trial registration: UNISC N374.298. Registered 04 JUN 2013 (retrospectively registered).
Gonçalves da Silva AL, Karnopp TE, Ferreira Weber A, Goulart C da L, Scheneiders P de B, Cardoso DM, et al. DNA damage and repair capacity in lymphocyte of chronic obstructive pulmonary diseases patients during physical exercise with oxygen supplementation. Multidiscip Respir Med [Internet]. 2016 Dec. 14 [cited 2024 Jul. 4];11(1). Available from: https://mrmjournal.org/index.php/mrm/article/view/349
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