Metabolomic profile of amniotic fluid to evaluate lung maturity: the diaphragmatic hernia lamb model
Metabolomic profile of amniotic fluid to evaluate lung maturity: the diaphragmatic hernia lamb model
Authors
Gloria Pelizzo
Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia
Maurizio Ballico
Department of Medical and Biological Sciences, University of Udine, Udine
Maria Chiara Mimmi
Department of Medical and Biological Sciences, University of Udine, Udine
José Louis Peirò
Cincinnati Fetal Center. Pediatric Surgery Division, CCHMC, Cincinnati, OH
Mario Marotta
Fetal Surgery Program, Congenital Malformations Research Group, Research Institute of Hospital Universitari Vall d’Hebron, Edifici Infantil, Barcelona
Costanzo Federico
Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia
Erika Andreatta
Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia
Ghassan Nakib
Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia
Maurilio Sampaolesi
Laboratory of Translational Cardiomyology, Stem Cell Interdepartmental Institute, KU Leuven, Belgium; Human Anatomy, University of Pavia, Pavia
Elisa Zambaiti
Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia
Valeria Calcaterra
Department of the Mother and Child Health, Pediatric Surgery Unit, Department of Internal Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Pavia
Background: Tracheal occlusion (TO) stimulates lung growth in fetuses affected with congenital diaphragmatic hernia (CDH) although the processes involved in lung maturation still remain unknown. The objective of this study was to evaluate the metabolomic profile of amniotic fluid (AF) following TO in fetal lamb model in order to obtain an indirect view of mechanisms involved in pulmonary reversal hypoplasia and biochemical maturity in response to fetal TO.
Methods: Liquid Chromatography Mass Spectrometry was performed on lamb AF samples at: age I (70 days’gestation); age II (102 days’ gestation); age III (136 days’ gestation). CDH was induced at age I and TO at age II.
Results: Betaine, choline, creatinine were found significantly increased during gestation in the control group. The CDH group showed choline (p =0.007) and creatinine (p =0.004) decreases during pregnancy. In the TO group choline and creatinine profiles were restored.
Conclusions: Alveolar tissue and fetal global growth ameliorated after TO. Metabolomics provided useful information on biochemical details during lung maturation. Metabolomic profiling would help to identify the best time to perform TO, in order to increase survival of CDH affected patients.
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